Catalog No: OKCD03562
Size:96WELLS
Price: $875.00
SKU
OKCD03562
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HNF1B Chemi-Luminescent ELISA Kit (Rat) (OKCD03562)

Datasheets/ManualsPrintable datasheet for HNF1B Chemi-Luminescent ELISA Kit (Rat) (OKCD03562)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Beta (HNF1b) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Hnf1b Microplate96 Wells (12 x 8 Well strips)
Hnf1b Lyophilized Standard2
100X Hnf1b Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHnf1b
Gene Full NameHNF1 homeobox B
Alias Symbolshepatocyte nuclear factor 1-beta;HNF-1B;HNF-1-beta;LF-B3;Tcf2;Transcription factor 2;Transcription factor 2, hepatic;variant hepatic nuclear factor 1;VHNF1.
NCBI Gene Id25640
Protein NameHepatocyte nuclear factor 1-beta
Description of TargetTranscription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Uniprot IDP23899
Protein Accession #NP_037235
Nucleotide Accession #NM_013103
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