HNF1A ELISA Kit (Human) (OKEH08105)

Catalog No: OKEH08105

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	90-99% (n = 20)	91-101% (n = 20)	101-111% (n = 20)	90-100% (n = 20)
  EDTA plasma(n=5)	105-114% (n = 20)	106-115% (n = 20)	94-104% (n = 20)	91-101% (n = 20)
  heparin plasma(n=5)	95-104% (n = 20)	98-108% (n = 20)	92-102% (n = 20)	103-115% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology HNF1A ELISA Kit (Human) (OKEH08105) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HNF1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HNF1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HNF1A captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=9.1% (n = 20)
  Mean Inter-assay CV%: <=9.3% (n = 20)
• ELISA Kit Component:
  • HNF1A Microplate: 96 Wells (12 x 8 Well strips)
  • HNF1A Lyophilized Standard: 2
  • 100X Biotinylated HNF1A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
  Subcellular Location: Nucleus
  Unigene: Hs.654455
  SMR: P20823
  String: 9606.ENSP00000257555
  KEGG: hsa:6927
  MIM: 142330
  Pfam: PF04814
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Human Hepatocyte nuclear factor 1-alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HNF1A
• Gene Full Name: HNF1 homeobox A
• Alias Symbols: albumin proximal factor;hepatic nuclear factor 1;hepatocyte nuclear factor 1-alpha;HNF1;HNF-1A;HNF1alpha;HNF-1-alpha;HNF4A;IDDM20;interferon production regulator factor;LFB1;liver-specific transcription factor LF-B1;MODY3;TCF1;TCF-1;Transcription factor 1;transcription factor 1, hepatic;truncated hepatocyte nuclear factor 1 alpha.
• NCBI Gene Id: 6927
• Protein Name: P20823
• Description of Target: Hepatocyte nuclear factor 1-alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P20823
• Protein Accession #: NP_000536.5
• Nucleotide Accession #: NM_000545.6