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Catalog No: OKCD02590
Size:96WELLS
Price: $675.00
SKU
OKCD02590
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Datasheets/ManualsPrintable datasheet for HNF1A ELISA Kit (Human) (OKCD02590)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
91-105%79-104%97-105%78-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Alpha (HNF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
92-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HNF1A Microplate96 Wells (12 x 8 Well strips)
HNF1A Lyophilized Standard2
100X Biotinylated HNF1A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Alpha (HNF1a). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Alpha (HNF1a) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolHNF1A
Gene Full NameHNF1 homeobox A
Alias Symbolsalbumin proximal factor;hepatic nuclear factor 1;hepatocyte nuclear factor 1-alpha;HNF1;HNF-1A;HNF1alpha;HNF-1-alpha;HNF4A;IDDM20;interferon production regulator factor;LFB1;liver-specific transcription factor LF-B1;MODY3;TCF1;TCF-1;Transcription factor 1;transcription factor 1, hepatic;truncated hepatocyte nuclear factor 1 alpha.
NCBI Gene Id6927
Protein NameHepatocyte nuclear factor 1-alpha
Description of TargetTranscriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Uniprot IDP20823
Protein Accession #NP_000536.5
Nucleotide Accession #NM_000545.6
Protein Size (# AA)631
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