HMOX1 ELISA Kit (Human) (OKBB00836)

Catalog No: OKBB00836

Size:96 wells

HMOX1 ELISA Kit (Human) (OKBB00836) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human HO-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HO-1 has been precoated onto 96-well plates. Standards(E.coli, M1-M288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HO-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human HO-1 amount of sample captured in plate.
• ELISA Kit Range: 93.7 pg/ml - 6,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	149	658	3760	138	610	3391
  Standard deviation	11.62	32.9	180.48	12.69	39.65	200.06
  CV(%)	7.8	5	4.8	9.2	6.5	5.9

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human HMOX1 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human HMOX1 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human HMOX1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	149	156	130	134	142	10.63	7.4%
  Sample 2	658	603	678	602	635	33.5	5.2%
  Sample 3	3760	3562	3498	3701	3630	104.86	2.8%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
  O.D.	0.020	0.063	0.111	0.201	0.383	0.845	1.465	2.276

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human HO-1
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: M1-M288
• Description: For quantitative detection of human HO-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: HMOX1
• Gene Full Name: heme oxygenase 1
• Alias Symbols: 32 kD, bK286B10, D8Wsu38e, heat shock protein 32 kD, heat shock protein 32kD, Heat shock protein, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, HMOX 1, Hmox, HMOX1, HMOX1_HUMAN, HO 1, HO, HO-1, HO1, Hsp32
• NCBI Gene Id: 3162
• Protein Name: Heme oxygenase 1
• Description of Target: HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
• Uniprot ID: P09601
• Protein Accession #: NP_002124.1
• Nucleotide Accession #: NM_002133.2