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| Datasheets/Manuals | Printable datasheet for HMGN1 Antibody (Phospho-Ser21) (OAAF00221) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | HMG14 (Phospho-Ser21) antibody detects endogenous levels of HMG14 only when phosphorylated at serine 21. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Dilution | The optimal working dilution should be determined by the end user. |
| Application Info | IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000 |
| Reference | Mihael H. Polymeropoulos. Nucleic Acids Res., Jul 1991; 19: 3753. LJ Arwood. J. Biol. Chem., Jun 1990; 265: 9771 - 9777. Thomas Hildmann. Genome Res., Apr 1999; 9: 360 - 372. |
| Gene Symbol | HMGN1 |
|---|---|
| Gene Full Name | high mobility group nucleosome binding domain 1 |
| Alias Symbols | high mobility group nucleosome-binding domain-containing protein 1;high-mobility group (nonhistone chromosomal) protein 14;high-mobility group nucleosome binding 1;HMG14;nonhistone chromosomal protein HMG-14;non-histone chromosomal protein HMG-14. |
| NCBI Gene Id | 3150 |
| Protein Name | Non-histone chromosomal protein HMG-14 |
| Description of Target | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity). |
| Uniprot ID | P05114 |
| Protein Accession # | NP_004956.5 |
| Molecular Weight | 10 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
This target may also be called "high mobility group nucleosome-binding domain-containing protein 1;high-mobility group (nonhistone chromosomal) protein 14;high-mobility group nucleosome binding 1;HMG14;nonhistone chromosomal protein HMG-14;non-histone chromosomal protein HMG-14." in publications.
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What is the shipping cost for "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "10 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HMGN1 Antibody (Phospho-Ser21) (OAAF00221)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HMGN1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HMGN1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HMGN1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HMGN1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HMGN1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HMGN1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
