Catalog No: OKCD07640
Size:96WELLS
Price: $750.00
SKU
OKCD07640
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Datasheets/ManualsPrintable datasheet for HMGCS1 ELISA Kit (Human) (OKCD07640)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
HMGCS1 Microplate96 Wells (12 x 8 Well strips)
HMGCS1 Lyophilized Standard2
100X HMGCS1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.053ng/mL
Gene SymbolHMGCS1
Gene Full Name3-hydroxy-3-methylglutaryl-CoA synthase 1
Alias Symbols3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase;3-hydroxy-3-methylglutaryl coenzyme A synthase;3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble);3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble);HMGCS;hydroxymethylglutaryl-CoA synthase, cytoplasmic.
NCBI Gene Id3157
Protein NameHydroxymethylglutaryl-CoA synthase, cytoplasmic
Description of TargetThis enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Uniprot IDQ01581
Protein Accession #NP_001091742.1
Nucleotide Accession #NM_001098272.2
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