Catalog No: OKEH01045
Price: $525.00
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HMGB2 ELISA Kit (Human) : 96 Wells (OKEH01045)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01045
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HMGB2 ELISA Kit (Human) (OKEH01045) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMGB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMGB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMGB2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
HMGB2 Microplate96 Wells (12 x 8 Well strips)
HMGB2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HMGB2 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Human High mobility group protein B2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHMGB2
Gene Full Namehigh mobility group box 2
Alias Symbolshigh mobility group protein 2;high mobility group protein B2;high-mobility group (nonhistone chromosomal) protein 2;HMG2;HMG-2.
NCBI Gene Id3148
Protein NameP26583
Description of TargetHigh mobility group protein B2
Uniprot ID
Protein Accession #NP_001124160.1
Nucleotide Accession #NM_001130688.1
Protein Size (# AA)209
Molecular Weight24 kDa
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