HMGB2 Antibody (OAGA07257)

Catalog No: OAGA07257

Size:100 ul

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody diluted at 1:1000.

Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody diluted at 1:2000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody diluted at 1:20000.

HMGB1/HMGB2 antibody detects HMGB2 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
E. 30 ug A375 whole cell lysate/extract
12 % SDS-PAGE
HMGB1/HMGB2 antibody dilution: 1:10000

HMGB1/HMGB2 antibody detects HMGB2 protein at nucleus on human duodenal adenocarcinoma tissue by immunohistochemical analysis.
Sample: Paraffin-embedded human duodenal adenocarcinoma tissue.
HMGB1/HMGB2 antibody dilution: 1:500.

HMGB1/HMGB2 antibody detects HMGB2 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGB1/HMGB2 antibody dilution: 1:500.

HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 1X PBS, 20% glycerol with 0.01% Thimerosal added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
  Positive Controls: 293T, A431, HeLa, HepG2, A375, mouse brain, rat brain
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Full length human HMGB1/HMGB2 Recombinant protein.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Xenopus laevis (87%), Pig (99%), Chicken (92%), Bovine (100%)
• Concentration: 1 mg/ml
• Application Info: ICC/IF: 1:100~2000
  IHC-FFPE: 1:100~1000
  WB: 1:500~20000

Target Info

• Gene Symbol: HMGB2
• Gene Full Name: high mobility group box 2
• Alias Symbols: HMG2
• NCBI Gene Id: 3148
• Protein Name: high mobility group protein B2
• Description of Target: This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.
• Uniprot ID: P26583
• Protein Accession #: NP_001124160.1
• Nucleotide Accession #: NM_001130688.1
• Molecular Weight: 24 kDa