HMGB1 Recombinant Protein (OPCD03915)

Catalog No: OPCD03915

HMGB1 Recombinant Protein (OPCD03915) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Ctrl (+), SDS-PAGE, WB
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Met1-Glu215
  Isoelectric Point: 5.6
  Subcellular Location: Nucleus, Cytoplasm, Chromosome
  
  Phenomenon Explanation: The possible reasons the actual band size differs from the predicted are as follows:
  1) Splice Variants: Alternative splicing may create different sized proteins from the same gene.
  2) Relative charge: The composition of amino acids may affect the charge of the protein
  3) Post-translational modification: Phosphorylation, glycosylation, methylation, etc.
  4) Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form.
  5) Polymerization of target protein: Dimerization, multimerization, etc.
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 90%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: Gm21596|Hmgb1
• Gene Full Name: high mobility group box 1|predicted gene, 21596
• Alias Symbols: amph;amphoterin;high mobility group protein 1;high mobility group protein B1;Hmg1;HMG-1;p3;p30;SBP;SBP-1.
• NCBI Gene Id: 100862258|15289
• Protein Name: High mobility group protein B1
• Description of Target: Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogenic activity (PubMed:16365390). May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins (By similarity).
• Uniprot ID: P63158
• Protein Accession #: NP_001300823.1
• Nucleotide Accession #: NM_001313894.1
• Protein Size (# AA): Met1~Glu215
• Molecular Weight: 33kDa