HMGB1 ELISA Kit (Mouse) (OKCD04072)

Catalog No: OKCD04072 (Formerly GWB-U3NO5F)

Size:96 Wells

HMGB1 ELISA Kit (Mouse) (OKCD04072) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Linearity:

  Matrix	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	78-92%	87-95%	94-102%
  EDTA Plasma (n=5)	82-90%	83-97%	80-88%	85-96%
  Heparin Plasma(n=5)	97-105%	86-104%	86-102%	95-105%

• ELISA Kit Principle: Aviva Systems Biology HMGB1 ELISA Kit (Mouse) (OKCD04072) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMGB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMGB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMGB1 captured in the well.
• ELISA Kit Range: 46.88-3,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	86-95	92
  EDTA Plasma (n=5)	90-104	101
  Heparin Plasma(n=5)	80-94	89

• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <10% (n = 20)
  Mean Inter-Assay Precision - <12% (n = 8)
• ELISA Kit Component:
  • HMGB1 Microplate: 96 Wells (12 x 8 Well strips)
  • HMGB1 Lyophilized Standard: 2 x 3 ng
  • 100X Biotinylated HMGB1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 18.29 pg/mL
• Publications:

  HMGB1 promotes ductular reaction and tumorigenesis in autophagy-deficient livers. J Clin Invest. 128, 2419-2435 (2018) 29558368

  Loss of High-Mobility Group Box 1 (HMGB1) Protein in Rods Accelerates Rod Photoreceptor Degeneration After Retinal Detachment. Invest Ophthalmol Vis Sci. 61, 50 (2020). 32460314

  Streptococcal sagA activates a proinflammatory response in mast cells by a sublytic mechanism. Cell Microbiol. 21, e13064 (2019). 31155820

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HMGB1
• Gene Full Name: high mobility group box 1
• Alias Symbols: p3, SBP, p30, Hmg1, amph, HMG-1, SBP-1
• NCBI Gene Id: 15289
• Protein Name: High mobility group protein B1
• Description of Target: Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury.
• Uniprot ID: P63158
• Protein Accession #: NP_065390
• Nucleotide Accession #: NM_000079