Catalog No: OKCD04074 (Formerly GWB-C6JUBT)
Size:96 Wells
Price: $600.00
SKU
OKCD04074
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit Linearity
Matrix1:21:41:81:16
Serum (n=5)99-105%91-101%82-97%81-104%
EDTA Plasma (n=5)95-102%89-97%85-96%95-105%
Heparin Plasma(n=5)91-98%80-97%80-93%98-105%
ELISA Kit PrincipleAviva Systems Biology HMGB1 ELISA Kit (Human) (OKCD04074) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMGB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMGB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMGB1 captured in the well.
ELISA Kit Range62.5-4,000 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)96-105101
EDTA Plasma (n=5)81-9589
Heparin Plasma(n=5)79-9084
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <10% (n = 20)
Mean Inter-Assay Precision - <12% (n = 8)
ELISA Kit Component
ComponentAmount
HMGB1 Microplate96 Wells (12 x 8 Well strips)
HMGB1 Lyophilized Standard2 x 3 ng
100X Biotinylated HMGB1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity28.3 pg/mL
Publications

Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study. J Clin Med. 9, (2020). 32629885

Dehydrocostus lactone suppresses ox-LDL-induced attachment of monocytes to endothelial cells. Am J Transl Res. 11, 6159-6169 (2019). 31632584

Synthetic lethality between VPS4A and VPS4B triggers an inflammatory response in colorectal cancer. EMBO Mol Med. 12, e10812 (2020). 31930723

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolHMGB1
Gene Full Namehigh mobility group box 1
Alias SymbolsHMG1, HMG3, HMG-1, SBP-1
NCBI Gene Id3146
Protein NameHigh mobility group protein B1
Description of TargetMultifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury.
Uniprot IDP09429
Protein Accession #NP_065390
Nucleotide Accession #NM_000079
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