HMGB1 Antibody (OAGA01541)

Catalog No: OAGA01541

Size:100 ul

HMGB1 antibody detects HMGB1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (OAGA01541) diluted by 1:3000.

HMGB1 antibody detects HMGB1 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug A375 whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody (OAGA01541) dilution: 1:3000

HMGB1 antibody detects HMGB1 protein by Western blot analysis.
A. 30 ug NIH-3T3 whole cell lysate/extract
B. 30 ug JC whole cell lysate/extract
C. 30 ug BCL-1 whole cell lysate/extract
D. 30 ug C2C12 whole cell lysate/extract
E. 30 ug Raw264.7 whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody (OAGA01541) dilution: 1:3000

HMGB1 antibody detects HMGB1 protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody (OAGA01541) dilution: 1:3000

HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 antibody (OAGA01541) dilution: 1:1000.

HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (OAGA01541) dilution: 1:1000.

HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
HMGB1 antibody (OAGA01541) dilution: 1:1000.

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using HMGB1 (OAGA01541) antibody at 1:500 dilution.

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (OAGA01541) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (OAGA01541) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Pig
• Product Format: Liquid 1XPBS, 20% glycerol (pH 7) with 0.01% Thimerosal added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC, IHC-FFPE, WB
  Positive Controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, C2C12, Raw264.7, PC-12
  Cellular Localization: 25 kDa
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Xenopus laevis (93%), Dog (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)
• Concentration: 0.37 mg/ml
• Application Info: WB: 1:500~10000
  ICC/IF: 1:100~1000
  IHC: Assay-dependent dilution
  IHC-FFPE: 1:100~1000

Target Info

• Gene Symbol: HMGB1
• Gene Full Name: high mobility group box 1
• Alias Symbols: HMG1, HMG3, HMG-1, SBP-1
• NCBI Gene Id: 3146
• Description of Target: DNA binding proteins that associate with chromatin and have the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
• Uniprot ID: P09429
• Molecular Weight: 25 kDa