HMGA1 ELISA Kit (Human) (OKEH04469)

Catalog No: OKEH04469

Size:96T

Human HMGA1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HMGA1 ELISA Kit (Human) (OKEH04469) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMGA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for HMGA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMGA1 captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.1% (n = 20)
  Mean Inter-assay CV%: <= 7.2% (n = 20)
• ELISA Kit Component:
  • Anti-HMGA1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HMGA1 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • HMGA1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.039 ng/mL
• Specificity: Natural and recombinant Human High mobility group protein HMG-I/HMG-Y
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HMGA1
• Gene Full Name: high mobility group AT-hook 1
• Alias Symbols: High mobility group AT-hook protein 1;high mobility group protein A1;high mobility group protein HMG-I/HMG-Y;high mobility group protein R;HMGA1A;HMGIY;HMG-R;nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y.
• NCBI Gene Id: 3159
• Protein Name: P17096
• Description of Target: High mobility group protein HMG-I/HMG-Y
• Uniprot ID: https://www.uniprot.org/uniprot/P17096
• Protein Accession #: NP_001306006.1
• Nucleotide Accession #: NM_001319077.1
• Protein Size (# AA): 107
• Molecular Weight: 12 KDa