Catalog No: OKEH08281
Size:96 Wells
Price: $725.00
SKU
OKEH08281
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HMCES ELISA Kit (Mouse) (OKEH08281)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology HMCES ELISA Kit (Mouse) (OKEH08281) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMCES has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMCES is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMCES captured in well.
ELISA Kit Range0.78 - 50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=6.8% (n = 20)
Mean Inter-assay CV%: <=8.1% (n = 20)
ELISA Kit Component
ComponentAmount
HMCES Microplate96 Wells (12 x 8 Well strips)
HMCES Lyophilized Standard2
100X Biotinylated HMCES Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (By similarity). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA (By similarity). The HMCES DNA-protein cross-link is then degraded by the proteasome (By similarity). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (By similarity). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (PubMed:29020633). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells (PubMed:23434322). May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (PubMed:29020633).
::Subcellular Location: Chromosome Recruited to chromatin following DNA damage. Localizes to replication forks.
::Unigene: Mm.246241
SMR: Q8R1M0
String: 10090.ENSMUSP00000032141
::KEGG: mmu:232210
Pfam: PF02586
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.23ng/mL
SpecificityNatural and recombinant Mouse Abasic site processing protein HMCES
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHMCES
Gene Full Name5-hydroxymethylcytosine (hmC) binding, ES cell specific
Alias SymbolsS, Srap1, C85376, 8430410A17Rik
NCBI Gene Id232210
Protein Nameabasic site processing protein HMCES
Swissprot IdQ8R1M0
Protein Accession #NP_776098.1
Nucleotide Accession #NM_173737.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express HMCES.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express HMCES.
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