HMCES ELISA Kit (Mouse) (OKEH08281)

Catalog No: OKEH08281

Size:96T

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology HMCES ELISA Kit (Mouse) (OKEH08281) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMCES has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMCES is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMCES captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=8.1% (n = 20)
• ELISA Kit Component:
  • HMCES Microplate: 96 Wells (12 x 8 Well strips)
  • HMCES Lyophilized Standard: 2
  • 100X Biotinylated HMCES Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (By similarity). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA (By similarity). The HMCES DNA-protein cross-link is then degraded by the proteasome (By similarity). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (By similarity). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (PubMed:29020633). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells (PubMed:23434322). May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (PubMed:29020633).
  Subcellular Location: Chromosome Recruited to chromatin following DNA damage. Localizes to replication forks.
  Unigene: Mm.246241
  SMR: Q8R1M0
  String: 10090.ENSMUSP00000032141
  KEGG: mmu:232210
  Pfam: PF02586
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.23ng/mL
• Specificity: Natural and recombinant Mouse Abasic site processing protein HMCES
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hmces
• Gene Full Name: 5-hydroxymethylcytosine (hmC) binding, ES cell specific
• Alias Symbols: 8430410A17Rik;abasic site processing protein HMCES;C85376;embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein;ES cell-specific 5hmC-binding protein;peptidase HMCES;putative endonuclease HMCES;putative peptidase SRAPD1;S;SRAP domain-containing protein 1;Srap1;UPF0361 protein C3orf37 homolog.
• NCBI Gene Id: 232210
• Protein Name: Q8R1M0
• Description of Target: Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q8R1M0
• Protein Accession #: NP_776098.1
• Nucleotide Accession #: NM_173737.2