HLA-G Antibody (OAEE01055)

Catalog No: OAEE01055

Size:100UG

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.

Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA-G (MEM-G/9) Alexa Fluor 488 Fab-fragment.

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-G/9
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
• Purification: Purified by size-exclusion chromatography
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
  The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
• Formulation: FITC
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Zhao L, Teklemariam T, Hantash BM: Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens. 2012 Sep;80(3):231-8
  Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb;32(2):311-5.
  Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8.
  Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74.
  Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A. Regulatory role of tryptophan degradation pathway in HLA-G expression by humanmonocyte-derived dendritic cells.Mol Immunol. 2006 Jul;43(14):2151-60.
  Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26.
  Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R: Lung macrophages and dendritic cells express HLA-G molecules in pulmonarydiseases. Hum Immunol. 2002 Feb;63(2):83-90.
  Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. AIDS. 2002 Feb 15;16(3):347-51.
  Abstracts from the 3rd International Conference on HLA-G. Tissue Antigens 62, 339-357 (2003).
  

Target Info

• Gene Symbol: HLA-G
• Gene Full Name: major histocompatibility complex, class I, G
• Alias Symbols: b2 microglobulin;HLA class I histocompatibility antigen, alpha chain G;HLA G antigen;HLA-G histocompatibility antigen, class I, G;MHC class I antigen G;MHC class Ib antigen;MHC-G;mutant MHC class I antigen;mutant MHC class Ib antigen.
• NCBI Gene Id: 3135
• Protein Name: HLA class I histocompatibility antigen, alpha chain G
• Description of Target: Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.
• Uniprot ID: P17693
• Protein Accession #: NP_002118.1
• Nucleotide Accession #: NM_002127.5