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Datasheets/Manuals | Printable datasheet for anti-HLA-F (ARP49411_P050-HRP) antibody |
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Predicted Species Reactivity | Human, Cow, Pig |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | IHC, WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human HLA-F |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 93%; Human: 100%; Pig: 100% |
Peptide Sequence | Synthetic peptide located within the following region: EAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADT |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-HLA-F (ARP49411_P050-HRP) antibody is Catalog # AAP49411 (Previous Catalog # AAPP29130) |
Sample Type Confirmation | HLA-F is strongly supported by BioGPS gene expression data to be expressed in 721_B |
Reference | Burfoot,R.K., (2008) Tissue Antigens 71 (1), 42-50 |
Gene Symbol | HLA-F |
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Gene Full Name | Major histocompatibility complex, class I, F |
Alias Symbols | HLAF, CDA12, HLA-5.4, HLA-CDA12 |
NCBI Gene Id | 3134 |
Protein Name | HLA class I histocompatibility antigen, alpha chain F |
Description of Target | HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. |
Uniprot ID | P30511 |
Protein Accession # | NP_001091949 |
Nucleotide Accession # | NM_001098479 |
Protein Size (# AA) | 442 |
Molecular Weight | 48kDa |
Protein Interactions | UBC; LILRB2; LILRB1; TAP1; HLA-F; B2M; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Cow, Pig".
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How long will it take to receive "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
This target may also be called "HLAF, CDA12, HLA-5.4, HLA-CDA12" in publications.
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What is the shipping cost for "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "48kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HLA-F Antibody - N-terminal region : HRP (ARP49411_P050-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HLA-F"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HLA-F"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HLA-F"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HLA-F"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HLA-F"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HLA-F"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.