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Catalog No: OKEH01124
Size:96T
Price: $525.00
SKU
OKEH01124
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HLA-E ELISA Kit (Human) : 96 Wells (OKEH01124)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HLA-E ELISA Kit (Human) (OKEH01124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HLA-E has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HLA-E is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HLA-E captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
HLA-E Microplate96 Wells (12 x 8 Well strips)
HLA-E Lyophilized Standard2
100X Biotinylated HLA-E Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human HLA class I histocompatibility antigen, alpha chain E
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHLA-E
Gene Full Namemajor histocompatibility complex, class I, E
Alias SymbolsHLA class I histocompatibility antigen, alpha chain E;HLA-6.2;MHC class I antigen E;MHC class Ib antigen;QA1.
NCBI Gene Id3133
Protein NameP13747
Description of TargetHLA class I histocompatibility antigen, alpha chain E
Uniprot IDhttps://www.uniprot.org/uniprot/P13747
Protein Accession #NP_005507.3
Nucleotide Accession #NM_005516.5
Protein Size (# AA)358
Molecular Weight40 kDa
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