HLA-DRB5 Antibody (OAGA07915)

Catalog No: OAGA07915

Size:100 ul

Raji whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRB5 antibody diluted at 1:5000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human HLA-DRB5. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 2.38 mg/ml
• Preservative: 0.025% ProClin 300
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Target Info

• Gene Symbol: HLA-DRB5
• Gene Full Name: major histocompatibility complex, class II, DR beta 5
• Alias Symbols: HLA-DRB5*
• NCBI Gene Id: 3127
• Protein Name: major histocompatibility complex, class II, DR beta 5
• Description of Target: HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Uniprot ID: Q30154
• Protein Accession #: NP_002116.2
• Nucleotide Accession #: NM_002125.3