HLA-DRB5 Antibody - middle region (OAAB06426)

Catalog No: OAAB06426

Size:400UL

Western blot analysis of HLA-DRB5 Antibody (Center) in HL-60 cell lysates (35ug/lane). HLA-DRB5 (arrow) was detected using the purified Pab.

HLA-DRB5 is strongly supported by BioGPS gene expression data to be expressed in HL60

HLA-DRB5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HLA-DRB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Publications:

  Molecular Characteristics of High-Dose Melphalan Associated Oral Mucositis in Patients with Multiple Myeloma: A Gene Expression Study on Human Mucosa. PLoS ONE. 12, e0169286 (2017). 28052121

  More...

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HLA-DRB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the Central region of human HLA-DRB5.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  HLA-DRB5 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: HLA-DRB5
• Alias Symbols: DR beta-5, DR2-beta-2, Dw2, HLA class II histocompatibility antigen, DR-5 beta chain, HLA-DRB5*, major histocompatibility complex, class II, DR beta 5, MHC class II antigen DRB5.
• NCBI Gene Id: 3127
• Protein Name: HLA class II histocompatibility antigen, DR beta 5 chain|major histocompatibility complex, class II, DR beta 5 precursor [Homo sapiens]
• Description of Target: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
• Uniprot ID: Q30154
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_002116.2
• Molecular Weight: 30056