Catalog No: OAAB18099
Size:400ul
Price: $450.00
SKU
OAAB18099
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsPrintable datasheet for OAAB18099
Product Info
Predicted Species ReactivityHuman
Product FormatLiquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
ClonalityPolyclonal
IsotypeRabbit Ig
HostRabbit
ApplicationWB
Reconstitution and StorageMaintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
ImmunogenThis HLA-DRB1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-137 amino acids from the Central region of human HLA-DRB1.
PurificationThis antibody is purified through a protein A column, followed by peptide affinity purification.
Application InfoIHC-P: 1:25
WB: 1:1000
ReferenceTonnelle C., et al.EMBO J. 4:2839-2847(1985).
Bell J.I., et al.Proc. Natl. Acad. Sci. U.S.A. 82:3405-3409(1985).
Coppin H.L., et al.J. Immunol. 144:984-989(1990).
Raymond C.K., et al.Genome Res. 15:1250-1257(2005).
von Salome J., et al.Immunogenetics 59:261-271(2007).
Gene SymbolHLA-DRB1
Alias SymbolsHLA class II histocompatibility antigen, DRB1-1 beta chain, MHC class II antigen DRB1*1, DR-1, DR1, HLA-DRB1
Description of TargetBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading.
Uniprot IDP04229, Q30154
Molecular Weight30 kDa
  1. What is the species homology for "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".

  3. What buffer format is "HLA-DRB1 Antibody - middle region (OAAB18099)" provided in?

    This item is provided in "Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    This target may also be called "HLA class II histocompatibility antigen, DRB1-1 beta chain, MHC class II antigen DRB1*1, DR-1, DR1, HLA-DRB1" in publications.

  5. What is the shipping cost for "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "30 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "HLA-DRB1 Antibody - middle region (OAAB18099)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "HLA-DRB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "HLA-DRB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "HLA-DRB1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "HLA-DRB1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "HLA-DRB1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "HLA-DRB1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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