HLA-DQB1 Antibody (OASA04711)

Catalog No: OASA04711 (Formerly GWB-8C115F)

Size:250UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: YE2/36-HLK
• Isotype: IgG2a
• Host: Rat
• Application: Flow cytometry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin
• Additional Information: Fusion Partners: Spleen cells from immunised AO rats were fused with cells of the rat Y3 Ag1.2.3 myeloma cell line.
  Preparation: Purified IgG prepared by affinity chromatography on Protein G
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 1.0 mg/ml
• Reconstitution and Storage: -20°C
• Immunogen: EHR-B Ramos cells.
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: HLA DR
• Application Info: Flow Cytometry:    1/50 - 1/100
  (1) Special conditions apply. - See [*]
• Protocol Information: Citation: 1: Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ.Immunohistochemical detection of T-cell subsets and other leukocytes inparaffin-embedded rat and mouse tissues with monoclonal antibodies. J HistochemCytochem. 1995 Mar;43(3):313-20. PubMed PMID: 7868861.
  Species: Mouse, Rat
  Experiment Name: Immunohistochemistry.
  Experiment Background: Immunohistochemical localization of T-cells, CD4+ T-cells, CD8+ T-cells, B-cells, activated lymphocytes, major histocompatibility complex (MHC) class I1 antigens, macrophages, dendritic cells, and granulocytes in rat and mouse tissue fued in periodate-lysine-paraformaldehyde (PLP) and embedded in paraffin.
  Experimental Steps: Slides with paraffin sections were defrosted and dried at 37C for 30 min, dewaxed in Histoclear (twice for 5 min), and rehydrated in 100% ethanol (twice for 5 min), 75% ethanol (5 min) and tapwater ( 5 min). Slides with frozen sections were defrosted and dried at RT for 1 hr. They were then fixed in cold acetone (10 min), allowed to dry at RT (10 min), and washed in tapwater (10 min). Endogenous peroxidase was blocked with 0.3% H2O2 in PBS (paraffin sections), or 0.3% H2O2 in 50% PBS:50% methanol (frozen sections) for 15 min at RT. After washing in PBS, paraffin sections (mouse tissue only) were digested with 0.0025% trypsin (Difco; Detroit, MI) in 0.1% calcium chloride, pH 7.8, for 0, 5, or 10 minutes, followed by washing in three changes of PBS. Blocking serum, secondary antibodies, and ABC reagents were obtained from Vector Labs (Peterborough, UK). Nonspecific binding sites were blocked with 1.5% normal rabbit serum in PBS (mouse tissue) or 1.5% normal horse serum in PBS (rat tissue) for 30 min at RT. Excess serum was removed, the sections circled with a waterproof pen (Dako; Glostrup, Denmark), and incubated with primary MAb at 4C overnight as specified here above. A single batch of each antibody was used for the study. Diluent was 0.1% BSA in PBS. Negative control slides were incubated in either diluent alone (mouse tissue) or an inappropriate MAb, OX 21 Wnst human complement factor I (; Oxford, UK) (rat tissue). After washing in PBS the slides were incubated with biotinylated rabbit anti rat IgG pre-adsorbed with mouse serum (for mouse tissue) or biotinylated horse anti-mouse IgG, pre-adsorbed with rat serum (for rat tissue), followed by avidin-biotinylated horseradish peroxidase complex (ABC) and diaminobenzidine (Sigma) according to the Vectastain protocol (Vector).
  Other Reagents Used: Lysine monohydrochloride, disodium hydrogen orthophosphate, paraformaldehyd, sodium periodate, sodium pentobarbital
  Number Of Protocols: 1
• Reference: 1. Brickell, P.M. et al. (1981) A monoclonal antibody to the HLA-DR product recognizes a polymorphic Ia determinant in mice. Immunology. 43: 493-501.
  2. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leucocytes in Paraffin-embedded Rat and Mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43(3): 313-320.
  3. Bellinghausen, I.et al. (2003) Production of interleukin-13 by human dendritic cells after stimulation with protein allergens is a key factor for induction of T helper 2 cytokines and is associated with activation of signal transducer and activator of transcription-6. Immunology. 108:167-176.
  

Target Info

• Gene Symbol: HLA-DQB1
• Alias Symbols: IDDM1, CELIAC1, HLA-DQB
• NCBI Gene Id: 3119
• Description of Target: RAT ANTI HUMAN HLA DR
  
• Uniprot ID: P01920
• Protein Size (# AA): 42