HLA-DQB1 Antibody (OASA00461)
|Predicted Species Reactivity||Cat|
|Product Format||Purified IgG - liquid|
|::||Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
Preservative Stabilisers: 0.05% - Sodium Azide (NaN3)
|::||Approx Protein Conc: IgG concentration 1.0mg/ml |
Buffer Solutions: Phosphate buffered saline pH7.2
|Reconstitution and Storage||Store at +4oC or at -20oC if preferred.|
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Replacement Item||This antibody may replace item sc-17963 from Santa Cruz Biotechnology.|
|Predicted Homology Based on Immunogen Sequence||Cat|
|Datasheets/Manuals||Printable datasheet for anti-HLA-DQB1 antibody - OASA00461|
|Specificity||MHC CLASS II|
|Application Info||ELISA : This product may be used in an indirect ELISA or as either a capture or detection antibody in a sandwich ELISA together with OASA00460 (clone PF6J-6D).|
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blot: This product detects band/s of approximately 29-31kDa on fractionated cat lymphocyte cell lysates under non-reducing conditions.
|Target Reference||1. Hunt, P. et al. (1995) Variable expression of major histocompatibility complex class II in the domestic cat. Res. Vet. Sci. 59: 195-200.|
|Alias Symbols||IDDM1, CELIAC1, HLA-DQB, HLA-DQB1|
|NCBI Gene Id||3119|
|Description of Target||MOUSE ANTI CAT MHC CLASS II|
|Protein Size (# AA)||229|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express HLA-DQB1.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express HLA-DQB1.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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