HLA-C ELISA Kit (Human) (OKWB00176)

Catalog No: OKWB00176

Size:96T

Human HLA-C ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-99%	87-103%	85-103%	85-97%
  EDTA Plasma (n=5)	84-101%	82-99%	82-94%	82-100%
  Heparin Plasma (n=5)	82-93%	80-98%	80-96%	80-99%

• ELISA Kit Principle: Aviva Systems Biology HLA-C ELISA Kit (Human) (OKWB00176) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HLA-C has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HLA-C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HLA-C captured in the well.
• ELISA Kit Range: 0.313-20ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	91-101	97
  EDTA Plasma (n=5)	89-94	92
  Heparin Plasma (n=5)	87-96	91

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • HLA-C Microplate: 96 Wells (12 x 8 Well strips)
  • HLA-C Lyophilized Standard: 2 x 20 ng
  • 100X HLA-C Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HLA-C
• Gene Full Name: major histocompatibility complex, class I, C
• Alias Symbols: D6S204;HLA class I histocompatibility antigen, C alpha chain;HLAC;HLA-C antigen;HLA-JY3;HLC-C;human leukocyte antigen-C alpha chain;major histocompatibility antigen HLA-C;MHC;MHC class I antigen Cw*7;MHC class I antigen heavy chain HLA-C;PSORS1.
• NCBI Gene Id: 3107
• Protein Name: HLA class I histocompatibility antigen, Cw-7 alpha chain
• Description of Target: Involved in the presentation of foreign antigens to the immune system.
• Uniprot ID: P10321