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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for HLA-A Antibody (OASA02873) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | BB7.2 |
| Isotype | IgG2b |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin |
| Application | Flow cytometry|Functional assay|Immunohistochemistry-Frozen|Immunoprecipitation |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Histology Positive Control Tissue: Tonsil Endotoxin Level: < 0.01 EU/ug. |
| Reconstitution and Storage | -20°C |
| Immunogen | Papain solubilized HLA-A2 |
| Purification | Protein A Purified |
| Predicted Homology Based on Immunogen Sequence | Human |
| Concentration | 1 mg/ml |
| Specificity | Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. |
| Application Info | FC: 10 ug/mL. Use 10 uL of the suggested working dilution to label 106 cells or 100 uL whole blood. FN: 5 - 10 ug/mL |
| Application Data | Application #1: Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2  Application #2: Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: Azide Free  Application #3: Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE  Application #4: Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:Alexa Fluor 488  Application #5: Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:FITC  |
| Reference | 1. Parham, P. and Brodsky, F. M. (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody wih specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299. 2. Hogan, K. T. and Brown, S. L. (1992) Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192. 3. Harig, S. et al. (2001) Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukoctye antigen-A2 binding residues. Blood. 98: 2999-3005. 4. Pantenburg, B. et al. (2010) Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HLA-A Antibody (OASA02873)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "HLA-A Antibody (OASA02873)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "HLA-A Antibody (OASA02873)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HLA-A Antibody (OASA02873)"?
This target may also be called "HLAA" in publications.
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What is the shipping cost for "HLA-A Antibody (OASA02873)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HLA-A Antibody (OASA02873)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HLA-A Antibody (OASA02873)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HLA-A Antibody (OASA02873)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HLA-A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HLA-A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HLA-A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HLA-A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HLA-A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HLA-A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
