HLA-A Antibody (OAEE00416)

Catalog No: OAEE00416

Size:100UG

Western blot of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-147
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: PHA-activated peripheral blood lymphocytes
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.
  The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Application Data: WB Non-reducing conditions.
  FC 1 ug/ml
• Reference: Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
  Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lckand Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8.
  Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V.: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6.
  

Target Info

• Gene Symbol: HLA-A
• Gene Full Name: HLA class I histocompatibility antigen, A-1 alpha chain
• Alias Symbols: HLAA
• NCBI Gene Id: 3105
• Protein Name: HLA class I histocompatibility antigen, A-1 alpha chain
• Uniprot ID: P30443
• Protein Accession #: NP_001229687.1
• Nucleotide Accession #: NM_001242758.1