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Catalog No: OKEH05232
Size:96T
Price: $525.00
SKU
OKEH05232
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology HJURP ELISA Kit (Human) (OKEH05232) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HJURP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HJURP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HJURP captured in the well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 112%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.7%, n = 20
Mean Inter-Assay Precision - <11.8%, n = 20
ELISA Kit Component
ComponentAmount
HJURP Microplate96 Wells (12 x 8 Well strips)
HJURP Lyophilized Standard2
100X Biotinylated HJURP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.314 ng/mL
SpecificityNatural and recombinant Human Holliday junction recognition protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHJURP
Gene Full NameHolliday junction recognition protein
Alias Symbols14-3-3-associated AKT substrate;FAKTS;fetal liver expressing gene 1;fetal liver-expressing gene 1 protein;hFLEG1;Holliday junction recognition protein;up-regulated in lung cancer 9;URLC9.
NCBI Gene Id55355
Protein NameQ8NCD3
Description of TargetHolliday junction recognition protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q8NCD3
Protein Accession #NP_001269891.1
Nucleotide Accession #NM_001282962.1
Protein Size (# AA)748
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