HJURP Antibody - C-terminal region (OAAB09715)

Catalog No: OAAB09715

Size:400ul

HJURP Antibody (C-term) (Cat. #OAAB09715) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HJURP antibody detected the HJURP protein (arrow).

HJURP is strongly supported by BioGPS gene expression data to be expressed in Jurkat

HJURP Antibody (C-term) (Cat. #OAAB09715)immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HJURP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HJURP Antibody (C-term) (Cat. #OAAB09715) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
  Cellular Location: Nucleus, nucleolus. Chromosome, centromere. Note=Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage
  Tissue Location: According to PubMed:17256767, highly expressed in the thymus with lower levels in the placenta, small intestine, liver, skeletal muscle, and colon. According to PubMed:17823411, highly expressed in testis, and at a relatively lower level in thymus and bone marrow. Significantly overexpressed in many lung cancer samples, compared with normal lung
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HJURP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-588 amino acids from the C-terminal region of human HJURP.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  HJURP is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Intended Use: HJURP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: HJURP
• Alias Symbols: FAKTS, URLC9, hFLEG1
• NCBI Gene Id: 55355
• Protein Name: Holliday junction recognition protein
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q8NCD3
• Protein Accession #: NP_060880.3
• Molecular Weight: 83539 Da