HIV-1 TAT Protein (OPPA01531)

Catalog No: OPPA01531 (Formerly GWB-DB08FA)

Size:1UG

Product Info

• Predicted Species Reactivity: Human Immunodeficiency Virus Type 1
• Product Format: 1x PBS & 0.05% glycerol. Physical appearance: Sterile Filtered colorless solution.
• Host: E. Coli
• Application: ELISA, WB
  Product Introduction: Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication and infectivity. The HIV-1 TAT is a molecule of 86 aminoacids encoded by two exons. The product of the first exon is sufficient for the transactivation of the HIV-1 promoter. Biotin molecules can be coupled easily to either antigens (such as avidin, streptavidin, Fc binding proteins like Protein A or G) or antibodies and they can be used in a variety of detection systems. Biotin can be conjugated to many proteins without significantly changing their biological activity. Biotin binds with high affinity to avidin, streptavidin and NeutrAvidin protein. Every single protein can react with several molecules of biotin, each of which, in turn, can bind a molecule of avidin. This greatly increases the sensitivity of many assay procedures. The avidin-biotin interaction is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The bound formation between biotin and avidin is very rapid and, once formed, withstands extremes of pH, organic solvents and other denaturing agents. The avidinbiotin complex can withstand up to 3 M guanidine HCl. Biotin can be released by 8 M guanidine HCl at pH 1.5, or by autoclaving.
  Product Description: HIV-1 TAT Biotin Recombinant- produced in E.coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids encoded by two exons and having chain having a molecular mass of 14kDa and was biotinylated with NHS biotin.
• Reconstitution and Storage: HIV-1 TAT biotin recombinant although stable at 4C for 1 week, should be stored desiccated below -18C. Please prevent freeze-thaw cycles.
• Purity: Greater than 90.0% as determined by HPLC analysis & SDS-PAGE.
• Specificity: Immunoreactive with all sera of HIV-1 infected individuals.
• Application Info: HIV-1 TAT Biotin protein biotinylation is determined by Western Blotting and ELISA analysis using streptavidin - HRP conjugated as a detection reagent. Free biotin is eliminated by dialysis against PBS. Protein concentration is determined using a BIORAD protein assay.

Target Info

• Gene Symbol: HIV-1 TAT Biotin
• Alias Symbols: HIV-1 TAT, Biotin, HIV-1 TAT Recombinant, Biotin Labeled
• Description of Target: Recombinant HIV-1 TAT, Biotin Labeled
• Protein Size (# AA): Recombinant