HIV-1 TAT Clade-D Protein (OPPA01901)

Catalog No: OPPA01901 (Formerly GWB-DD91C5)

Size:2UG

Product Info

• Predicted Species Reactivity: Human Immunodeficiency Virus Type 1
• Product Format: Lyophilized with 10% glycerol. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Application: WB, SDS-PAGE
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication (Jones KA, 1994) and infectivity (Arya SK, 1985; Fisher AG, 1986). In addition, during acute infection, Tat is released extracellularly by infected cells (Chang HC, 1997; Ensoli B, 1990) and is taken up by neighboring cells where it transactivates viral replication (Ensoli B, 1993) and increases virus infectivity. HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.
  Product Description: HIV-1 TAT Clade-D Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain having the Accession number: AY194029.
• Reconstitution and Storage: Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18C. Upon reconstitution HIV-1 TAT should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Application Info: Western Blotting, SDS Page.Reacts with Mouse anti-Tat antibodies.

Target Info

• Gene Symbol: HIV-1 TAT Clade-D
• Alias Symbols: HIV-1 TAT Clade-D, HIV-1 TAT Clade-D Recombinant
• Description of Target: Recombinant HIV-1 TAT Clade-D
• Protein Size (# AA): Recombinant