HIV-1 Protein (OPPA00991)

Catalog No: OPPA00991 (Formerly GWB-96AB7E)

Size:100UG

Product Info

• Predicted Species Reactivity: Human Immunodeficiency Virus Type 1
• Product Format: 100 mM Na-PO4, pH 6 & 0.05% SDS. Physical appearance: Sterile filtered colorless clear solution.
• Host: E. Coli
• Application: ELISA, WB
  Product Introduction: HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids.Immunodeficiency develops more slowly with HIV-2.HIV-2 is less infectious in the early stages of the virus than with HIV-1.The infectiousness of HIV-2 increases as the virus progresses.Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion.HIV-1 gp41 helical regions can form more stable 6-helix bundles than HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25 degree C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.
  Product Description: HIV-1,2 recombinant- E.Coli derived recombinant 27 kDa protein contains the C- terminus of gp120 and most of gp41. The protein is conjugated to a 23 amino acids synthetic peptide derived from gp39 of HIV-2.
• Reconstitution and Storage: HIV-1,2 protein is shipped at ambient temperature. Upon arrival, Store at -20C Stable for Five years frozen. One month in solution at room temperature.
• Purity: Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
• Specificity: Immunoreactive with all sera of HIV-1, HIV type O & HIV-2 infected individuals.
• Application Info: HIV-1,2 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Target Info

• Gene Symbol: HIV-1, HIV-2
• Alias Symbols: HIV-1, HIV-2, HIV-1 envelope conjugated to HIV-2 gp39 Recombinant
• Description of Target: Recombinant HIV-1 Envelope conjugated to HIV-2 gp39
• Protein Size (# AA): Recombinant