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More Information
Predicted Species Reactivity Human
Product Format Liquid. Whole antiserum from rabbit containing 0.05% azide.
Clonality Polyclonal
Host Rabbit
Conjugation Unconjugated
Application CHIP, DB, WB, IF, IP
Reconstitution and Storage Store at -20C. Avoid repeated freeze/thaw cycles.
Replacement Item This antibody may replace item sc-105519 from Santa Cruz Biotechnology.
Purification Whole antiserum
Concentration Undetermined, ascites
Datasheets/Manuals Printable datasheet for anti-HIST3H3 (OADC00131) antibody
Preservative 0.05% Sodium Azide
Target Post-Translational Modification Phospho-Ser10
Specificity Polyclonal antibody raised in rabbit against histone H3 containing the phosphorylated serine 10 (H3S10p), using a KLH-conjugated synthetic peptide.
Application Info CHIP: 1 ul/CHIP
ELISA: 1:1,000 - 1:5,000
Dot Blotting: 1:20,000
Western Blotting: 1:500
Immunofluorescence: 1:200
Immunoprecipitation: 5 ul/IP
Gene Symbol HIST3H3
Official Gene Full Name histone cluster 3, H3
Alias Symbols H3t, H3.4, H3/g, H3FT
NCBI Gene Id 8290; 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Protein Name histone H3.1t
Description of Target Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Swissprot Id P68431
Protein Accession # NP_003520.1
Nucleotide Accession # NM_003529.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express HIST3H3.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express HIST3H3.
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