HIST2H3C2 ELISA Kit (Rat) (OKWB00376)

Catalog No: OKWB00376

Size:96T

Rat HIST2H3C2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-104%	85-100%	87-98%	87-105%
  EDTA Plasma (n=5)	83-101%	84-95%	82-99%	82-101%
  Heparin Plasma (n=5)	82-94%	85-99%	82-98%	83-94%

• ELISA Kit Principle: Aviva Systems Biology Hist2h3c2 ELISA Kit (Rat) (OKWB00376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hist2h3c2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hist2h3c2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hist2h3c2 captured in the well.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-105	97
  EDTA Plasma (n=5)	87-99	92
  Heparin Plasma (n=5)	85-100	94

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Hist2h3c2 Microplate: 96 Wells (12 x 8 Well strips)
  • Hist2h3c2 Lyophilized Standard: 2 x 1 ng
  • 100X Hist2h3c2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LOC102548682
• Gene Full Name: histone H4-like
• Alias Symbols: histone H3;uncharacterized protein LOC102548682.
• NCBI Gene Id: 102548682
• Protein Name: Histone H3
• Uniprot ID: D3ZJ08
• Protein Accession #: NP_001101168
• Nucleotide Accession #: NM_001107698