Hist1h4b ELISA Kit (Rat) (OKEH04804)

Catalog No: OKEH04804

Size:96T

Rat Hist1h4b ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Hist1h4b ELISA Kit (Rat) (OKEH04804) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hist1h4b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hist1h4b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hist1h4b captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Hist1h4b Microplate: 96 Wells (12 x 8 Well Strips)
  • Hist1h4b Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Hist1h4b Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.094 ng/mL
• Specificity: Natural and recombinant Rat Histone H4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: H4c3, Hist1h2ao, Hist1h4b, Hist1h4m, Hist2h4, Hist2h4a, LOC100912564, RGD1562378
• Gene Full Name: H4 clustered histone 3, histone cluster 1 H4 family member B, histone cluster 1, H2ao, histone cluster 1, H4m, histone cluster 2 H4 family member A, histone cluster 2, H4, histone H4 variant H4-v.1, histone H4-like
• Alias Symbols: germinal histone H4;H1ft;H4c2;H4f16;Hist1h4b;Hist4;Hist4h4;histone 1, H2ao;histone 1, H4m;histone 2, H4;histone cluster 1, H4b;histone cluster 2, H4;histone H2A type 1-E;histone H4;histone H4-like;RGD1565073.
• NCBI Gene Id: 100912489, 100912564, 291152, 295277, 364723, 500351, 502913, 64627
• Protein Name: P62804
• Description of Target: Histone H4
• Uniprot ID: https://www.uniprot.org/uniprot/P62804
• Protein Accession #: NP_001116941.1
• Nucleotide Accession #: NM_001123469.1
• Protein Size (# AA): 103
• Molecular Weight: 11 kDa