HIST1H46L2 ELISA Kit (Chicken) (OKEH08054)

Catalog No: OKEH08054

Size:96T

Chicken HIST1H46L2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 ELISA Kit (Chicken) (OKEH08054) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.0% (n = 20)
  Mean Inter-assay CV%: <=6.2% (n = 20)
• ELISA Kit Component:
  • HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 Microplate: 96 Wells (12 x 8 Well strips)
  • HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 Lyophilized Standard: 2
  • 100X Biotinylated HIST1H46L2, HIST1H4I, HIST1H46, HIST2H4B, HIST1H4D, HIST1H4B, HIST1H46L6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  Subcellular Location: Nucleus Chromosome
  Unigene: Gga.39882
  SMR: P62801
  String: 9031.ENSGALP00000037292
  KEGG: gga:100858049
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.056ng/mL
• Specificity: Natural and recombinant Chicken Histone H4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HIST1H46, HIST1H46L2, HIST1H46L6, HIST1H4B, HIST1H4D, HIST1H4I, HIST2H4B
• Gene Full Name: histone cluster 1, H4-IV, germinal H4-like 2
• Alias Symbols: dJ221C16.9;germinal histone H4;H1;H4;H4 (aa 1-103);H4 histone family, member A;H4 histone family, member B;H4/b; H4FB;H4/o;H4FA;H4-I;H4-II;H4-III;H4-IV;H4-V;H4-VI;H4-VII;H4-VIII;HIST1H46L1;HIST1H46L3;HIST1H46L4;HIST1H47;HIST1H4A;histone 1, H4;histone 1, H4a;histone 1, H4d;histone 2, H4b;histone cluster 1, H4a;histone cluster 1, H4-IV, germinal H4-like 1 (similar to human histone cluster 1, class H4 genes);histone cluster 1, H4-IV, germinal H4-like 2;histone cluster 1, H4-IV, germinal H4-like 2 (similar to human histone cluster 1, class H4 genes);histone cluster 1, H4-IV, germinal H4-like 3 (similar to human histone cluster 1, class H4 genes);histone cluster 1, H4-IV, germinal H4-like 4 (similar to human histone cluster 1, class H4 genes);histone cluster 1, H4-VI, germinal H4 (similar to human histone cluster 1, class H4 genes);histone cluster 1, histone class H4, member 7 (similar to human histone cluster 1, class H4 genes);histone H4;histone H4 (aa 1-103);histone H4 type VIII;histone H4-VI;histone H4-VII;histone H4-VIII;istone H4.
• NCBI Gene Id: 100858049, 100858319, 417946, 417950, 427884, 770005, 770142
• Protein Name: P62801
• Description of Target: Histone H4
• Uniprot ID: https://www.uniprot.org/uniprot/P62801
• Protein Accession #: NP_001032932.1
• Nucleotide Accession #: NM_001037843.1