HIST1H3A Antibody (OAAF00740)

Catalog No: OAAF00740 (Formerly GWB-ASC144)

Size:100 ug

Western blot analysis of extracts from HuvEc cells, treated with Serum 20% 30', using Histone H3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H3 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: Histone H3 (Ab-28) antibody detects endogenous levels of total Histone H3 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Zhong R., Nucleic Acids Res. 11:7409-7425(1983).
  Marashi F., Biochem. Cell Biol. 64:277-289(1986).
  Albig W., Genomics 10:940-948(1991)

Target Info

• Gene Symbol: H3-3A|H3-3B|H3C1|H3C10|H3C11|H3C12|H3C13|H3C14|H3C15|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8
• Gene Full Name: H3 clustered histone 1|H3 clustered histone 10|H3 clustered histone 11|H3 clustered histone 12|H3 clustered histone 13|H3 clustered histone 14
• Alias Symbols: H3;H3 histone family member 3A;H3 histone family member 3B;H3 histone family, member A;H3 histone family, member B;H3 histone family, member C;H3 histone family, member D;H3 histone family, member F;H3 histone family, member H;H3 histone family, member I;H3 histone family, member J;H3 histone family, member K;H3 histone family, member L;H3 histone family, member M;H3 histone, family 2;H3 histone, family 3A;H3 histone, family 3B (H3.3B);H3.1;H3.2;H3.3A;H3.3B;H3.f;H3/A;H3/b;H3/c;H3/d;H3/f;H3/h;H3/i;H3/j;H3/k;H3/l;H3/M;H3/n;H3/o;H3-3A;H3-3B;H3C1;H3C10;H3C11;H3C12;H3C13;H3C14;H3C15;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3-clustered histone 13;H3-clustered histone 14;H3-clustered histone 15;H3F1K;H3F2;H3F3;H3F3A;H3F3B;H3FA;H3FB;H3FC;H3FD;H3FF;H3FH;H3FI;H3FJ;H3FK;H3FL;H3FM;H3FN;HIST1H3A;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;HIST2H3A;HIST2H3C;HIST2H3D;histone 1, H3a;histone 1, H3b;histone 1, H3c;histone 1, H3d;histone 1, H3e;histone 1, H3f;histone 1, H3g;histone 1, H3h;histone 1, H3i;histone 1, H3j;histone 2, H3a;histone 2, H3c;histone 2, H3d;histone cluster 1 H3 family member a;histone cluster 1 H3 family member b;histone cluster 1 H3 family member c;histone cluster 1 H3 family member d;histone cluster 1 H3 family member e;histone cluster 1 H3 family member f;histone cluster 1 H3 family member g;histone cluster 1 H3 family member h;histone cluster 1 H3 family member i;histone cluster 1 H3 family member j;histone cluster 1, H3a;histone cluster 1, H3b;histone cluster 1, H3c;histone cluster 1, H3d;histone cluster 1, H3e;histone cluster 1, H3f;histone cluster 1, H3g;histone cluster 1, H3h;histone cluster 1, H3i;histone cluster 1, H3j;histone cluster 2 H3 family member a;histone cluster 2 H3 family member c;histone cluster 2 H3 family member d;histone cluster 2, H3a;histone cluster 2, H3c;histone cluster 2, H3d;histone H3.1;histone H3.2;histone H3.3;histone H3/a;histone H3/b;histone H3/c;histone H3/d;histone H3/f;histone H3/h;histone H3/i;histone H3/j;histone H3/k;histone H3/l;histone H3/m;histone H3/o.
• NCBI Gene Id: 126961|3020|3021|333932|653604|8350|8351|8352|8353|8354|8355|8356|8357|8358|8968
• Protein Name: Histone H3.1|Histone H3.2|Histone H3.3
• Description of Target: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Uniprot ID: P68431|P84243|Q71DI3
• Protein Accession #: NP_003520.1
• Molecular Weight: 15 kDa