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Catalog No: OCOA08931
Size:20UG
Price: $179.00
SKU
OCOA08931
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HIST1H2AG Protein Lysate (OCOA08931)

Datasheets/ManualsPrintable datasheet for HIST1H2AG Protein Lysate (OCOA08931)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionHIST1H2AG HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolH2AC11, H2AC13, H2AC15, H2AC16, H2AC17
Gene Full NameH2A clustered histone 11, H2A clustered histone 13, H2A clustered histone 15, H2A clustered histone 16, H2A clustered histone 17
Alias SymbolsdJ193B12.1;dJ193B12.9;H2A histone family, member C;H2A histone family, member D;H2A histone family, member I;H2A histone family, member N;H2A histone family, member P;H2A.1;H2A.1b;H2A.i;H2A/c;H2A/d;H2A/i;H2A/n;H2A/p;H2AC11;H2AC13;H2AC14;H2AC15;H2AC16;H2AC17;H2AFC;H2AFD;H2AFI;H2AFN;H2AFP;H2AG;HIST1H2AG;HIST1H2AI;HIST1H2AK;HIST1H2AL;HIST1H2AM;histone 1, H2ag;histone 1, H2ai;histone 1, H2ak;histone 1, H2al;histone 1, H2am;histone cluster 1 H2A family member g;histone cluster 1 H2A family member i;histone cluster 1 H2A family member k;histone cluster 1 H2A family member l;histone cluster 1 H2A family member m;histone cluster 1, H2ag;histone cluster 1, H2ai;histone cluster 1, H2ak;histone cluster 1, H2al;histone cluster 1, H2am;histone H2A type 1;histone H2A type 1-J;histone H2A/p;histone H2A/ptl;pH2A/f.
NCBI Gene Id8329, 8330, 8332, 8336, 8969
Protein NameA4FTV9, B2R5B3, NM_021064, NP_066408, P0C0S8
Description of TargetHistone H2A, Histone H2A type 1, histone H2A type 1 [Homo sapiens], Homo sapiens histone cluster 1 H2A family member g (HIST1H2AG), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P0C0S8
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_066408
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_021064
Molecular Weight14.1 kDa
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