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His Tag Antibody (OAEA00010)

50 ug
$49.00
In Stock
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Alias Symbols:
His Tag
Host:
Mouse
Clonality:
Monoclonal
Application:
IF, WB, DB, ELISA, IP, IS
Immunogen:
HHHHHH (6x His) synthetic peptide conjugated to KLH
Product Format:
50 ug at 1mg/mL in 10mM PBS, pH 7.2
Reconstitution and Storage:
Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store at 4C (add 0.1% NaN3) for several days to weeks, and at -20C for longer periods. 
Datasheets/Manuals:
Printable datasheet for OAEA00010
Specificity:
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Clone:
HIS.H8
Purity:
Protein A affinity chromatography from mouse ascites fluid
Isotype:
IgG2b
:::
Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins
Publications:

Leitman, J., Ulrich Hartl, F. & Lederkremer, G. Z. Soluble forms of polyQ-expanded huntingtin rather than large aggregates cause endoplasmic reticulum stress. Nat. Commun. 4, 2753 (2013). IF, WB, DB, ELISA, IP, IS, 24217578

Maklashina, E; Rajagukguk, S; Starbird, CA; McDonald, WH; Koganitsky, A; Eisenbach, M; Iverson, TM; Cecchini, G; Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. 291, 2904-16 (2016). IF, WB, DB, ELISA, IP, IS, 26644464

Shashikanth, N; Kisting, MA; Leckband, DE; Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions. 6, 23623 (2016). IF, WB, DB, ELISA, IP, IS, 27009566

Protocol Information:
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

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