Size:50 ug
$49.00
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SKU
OAEA00010
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His Tag Antibody (OAEA00010)

Catalog#: OAEA00010
Domestic: within 24 hours delivery | International: 3-5 days
More Information
Product Format Liquid 10mM PBS (pH 7.2) with 0.05% sodium azide
Clonality Monoclonal
Clone HIS.H8
Isotype IgG2b
Host Mouse
Application IF, WB, DB, IP, IS, ELISA
:: Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins
Reconstitution and Storage Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks, and at -20°C for 2 years or more.
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Purification Protein A affinity chromatography from mouse ascites fluid
Concentration 1 mg/ml
Datasheets/Manuals Printable datasheet for OAEA00010
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Application Info WB (ECL): 1:1000~3000 (incubate for one hour at room temperature)
IP: 1:50~200
IS: 1:500~2000
For best results with other assays (e.g.: DB, ELISA, etc), please determine optimal working dilution by titration test.
Other Applications Image 1 Data untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Publications

Leitman, J., Ulrich Hartl, F. & Lederkremer, G. Z. Soluble forms of polyQ-expanded huntingtin rather than large aggregates cause endoplasmic reticulum stress. Nat. Commun. 4, 2753 (2013). IF, WB, DB, ELISA, IP, IS, 24217578

Maklashina, E; Rajagukguk, S; Starbird, CA; McDonald, WH; Koganitsky, A; Eisenbach, M; Iverson, TM; Cecchini, G; Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. 291, 2904-16 (2016). IF, WB, DB, ELISA, IP, IS, 26644464

Shashikanth, N; Kisting, MA; Leckband, DE; Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions. 6, 23623 (2016). IF, WB, DB, ELISA, IP, IS, 27009566

Protocol Information WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Alias Symbols His Tag
  1. What is the species homology for "His Tag Antibody (OAEA00010)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "".

  2. How long will it take to receive "His Tag Antibody (OAEA00010)"?

    This item is available "Domestic: within 24 hours delivery | International: 3-5 days".

  3. What buffer format is "His Tag Antibody (OAEA00010)" provided in?

    This item is provided in "Liquid 10mM PBS (pH 7.2) with 0.05% sodium azide".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "His Tag Antibody (OAEA00010)"?

    This target may also be called "His Tag" in publications.

  5. What is the shipping cost for "His Tag Antibody (OAEA00010)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "His Tag Antibody (OAEA00010)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "His Tag Antibody (OAEA00010)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "His Tag Antibody (OAEA00010)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with ""?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with ""?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with ""?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with ""?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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