His Tag Antibody (OAEA00010)

Catalog No: OAEA00010

Size:50ug

Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5?g/mL)

Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)

Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).

Product Info

• Product Format: Liquid 10mM PBS (pH 7.2) with 0.05% sodium azide
• Clonality: Monoclonal
• Clone: HIS.H8
• Isotype: IgG2b
• Host: Mouse
• Application: IF, WB, DB, IP, IS, ELISA
  Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins
• Reconstitution and Storage: Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks, and at -20°C for 2 years or more.
• Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH
• Purification: Protein A affinity chromatography from mouse ascites fluid
• Concentration: 1 mg/ml
• Specificity: Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
• Application Info: WB (ECL): 1:1000~3000 (incubate for one hour at room temperature)
  IP: 1:50~200
  IS: 1:500~2000
  For best results with other assays (e.g.: DB, ELISA, etc), please determine optimal working dilution by titration test.
• Other Applications Image 1 Data: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
• Publications:

  Ablation of Ggnbp2 impairs meiotic DNA double-strand break repair during spermatogenesis in mice. J Cell Mol Med. 22, 4863-4874 (2018). 30055035

  Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors. J Biol Chem. 290, 15909-20 (2015). 25944910

  Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. J. Biol. Chem. 291, 2904-16 (2016). 26644464

  Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products: multimerization, protein priming, and nucleotide preference. Curr Genet. 64, 1287-1301 (2018) 29761210

  JiHong Li, et al. Evidence That VirS Is a Receptor for the Signaling Peptide of the Clostridium perfringens Agr-like Quorum Sensing System. (2020). 32934089

  Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions. Sci Rep. 6, 23623 (2016). 27009566

  Leitman, J., Ulrich Hartl, F. & Lederkremer, G. Z. Soluble forms of polyQ-expanded huntingtin rather than large aggregates cause endoplasmic reticulum stress. Nat. Commun. 4, 2753 (2013). 24217578

  The N-Acetylglucosamine Kinase from Yarrowia lipolytica Is a Moonlighting Protein. Int J Mol Sci. 22 (2021). 34884915

  The unassembled flavoprotein subunits of human and bacterial complex II have impaired catalytic activity and generate only minor amounts of ROS. J Biol Chem. 293, 7754-7765 (2018). 29610278

Target Info

• Alias Symbols: His Tag