HIS Tag Antibody (OAAB06612)

Catalog No: OAAB06612

Size:200ul

HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody (6AT18).

Western blot analysis of lysates from 12tag protein , This demonstrates the His tagged antibody detected the His tagged protein (arrow).

Western blot analysis of lysate from 12 tag recombinant protein, using HIS Tag(Cat. #OAAB06612). OAAB06612 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Product Info

• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 6AT18
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000
  WB: 1:1,000
• Intended Use: HIS Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Target Info

• Description of Target: Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.