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HIF1A ELISA Kit (Pig) (OKCD00167)

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Application:
ELISA
Gene Symbol:
HIF1A
NCBI Gene Id:
396696
Alias Symbols:
HIF1-A, MOP1, PASD8, Basic Helix-Loop-Helix Transcription Factor, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78
Protein Name:
hypoxia-inducible factor 1-alpha
Swissprot Id:
A0T1H6
Protein Accession #:
NP_001116596
Nucleotide Accession #:
NM_001123124.1
Predicted Species Reactivity:
Pig
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
< 5.5 pg/mL
Kit Range:
15.62-1,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level HIF1A were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level HIF1A were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology HIF1A ELISA Kit (Pig) (OKCD00167) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF1A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HIF1Acaptured in well.
Kit Component:
ComponentAmount
Anti-HIF1A Microplate96 Wells (12 x 8 Well Strips)
HIF1A Lyophilized Standard2 x 1 ng
100X Biotinylated HIF1A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)87-97%78-103%89-97%91-98%
EDTA plasma(n=5)94-102%79-101%86-94%88-98%
heparin plasma(n=5)81-102%82-97%79-102%78-102%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)91-10196
EDTA plasma(n=5)84-9990
heparin plasma(n=5)88-10194
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HIF1A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HIF1A.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
824

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