HIF1A ELISA Kit (Pig) (OKCD00167)

Catalog No: OKCD00167 (Formerly GWB-KBBHR3)

Size:96WELLS

Pig HIF1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-97%	78-103%	89-97%	91-98%
  EDTA plasma(n=5)	94-102%	79-101%	86-94%	88-98%
  heparin plasma(n=5)	81-102%	82-97%	79-102%	78-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha (HIF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha (HIF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-101	96
  EDTA plasma(n=5)	84-99	90
  heparin plasma(n=5)	88-101	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HIF1A Microplate: 96 Wells (12 x 8 Well Strips)
  • HIF1A Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated HIF1A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 5.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HIF1A
• Gene Full Name: hypoxia inducible factor 1 subunit alpha
• Alias Symbols: hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1 alpha;hypoxia-inducible factor 1-alpha.
• NCBI Gene Id: 396696
• Protein Name: Hypoxia-inducible factor 1alpha
• Description of Target: Hypoxia-inducible factor 1alpha
• Uniprot ID: A0T1H6
• Protein Accession #: NP_001116596
• Nucleotide Accession #: NM_001123124.1
• Protein Size (# AA): 824