HIF1A Chemi-Luminescent ELISA Kit (Pig) (OKCD03559)

Catalog No: OKCD03559

Size:96WELLS

Pig HIF1A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-98%	78-101%	94-101%	88-103%
  EDTA Plasma (n=5)	89-103%	88-103%	93-101%	80-95%
  Heparin Plasma (n=5)	89-102%	78-89%	91-105%	88-95%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hypoxia Inducible Factor 1 Alpha (HIF1a) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	83-95	90
  Heparin Plasma (n=5)	78-98	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Hif1a Microplate: 96 Wells (12 x 8 Well strips)
  • Hif1a Lyophilized Standard: 2
  • 100X Hif1a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HIF1A
• Gene Full Name: hypoxia inducible factor 1 subunit alpha
• Alias Symbols: hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1 alpha;hypoxia-inducible factor 1-alpha.
• NCBI Gene Id: 396696
• Protein Name: Hypoxia-inducible factor 1alpha
• Description of Target: Hypoxia-inducible factor 1alpha
• Uniprot ID: A0T1H6
• Protein Accession #: NP_001116596
• Nucleotide Accession #: NM_001123124