HIF1A Antibody (OAGA07219)

Catalog No: OAGA07219

Size:100UL

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis.
A. 30 ug HepG2 whole cell lysate/extract (untreated)
B.30 ug HepG2 whole cell lysate/extract ( 200 uM CoCl2 treatment for 24 hr)
C. 30 ug HepG2 whole cell lysate/extract (500 uM CoCl2 treatment for 24 hr)
7.5% SDS-PAGE
HIF1 alpha antibody dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody diluted at 1:1000.

HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody dilution: 1:500.

HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis.
Samples: Untreated (left panel) and 500uM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min
Green: HIF1alpha protein stained by HIF1 alpha antibody diluted at 1:1000
Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody diluted at 1:2500
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Images captured by Olympus FV10i Confocal Laser Scanning Microscope

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody diluted at 1:200.
Blue: Hoechst 33342 staining.

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 um.

Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 uM CoCl2 for 24 hr using 5 ug of HIF1 alpha antibody .
Western blot analysis was performed using HIF1 alpha antibody .
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rabbit|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: CHIP|ICC|IF|IHC-Fr|IHC-P|IP|WB
• Additional Information: Positive Controls: MCF-7 , HeLa(500uM CoCl for 24hr) , NIH-3T3 (500 uM CoCl2 treatment for 24 hr) , HepG2 whole cell lysate/extract (1% O2 treatment for 24hr) , 293T transfected HIF1A , HepG2 ( 200 uM CoCl2 treatment for 24 hr) , HepG2 (500 uM CoCl2 treatment for 24 hr)
  Cellular Localization: Cytoplasm , Nucleus
• Reconstitution and Storage: -20°C or -80°C|2°C to 8°C
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog (97%), Pig (95%), Rabbit (95%)
• Concentration: 1.38 mg/ml
• Application Info: ICC/IF 1:100 - 1:1000
  IHC-FFPE 1:100 - 1:1000
  IP 1:100 - 1:570
  WB 1:500 - 1:3000
  

Target Info

• Gene Symbol: HIF1A
• Gene Full Name: hypoxia inducible factor 1 alpha subunit
• Alias Symbols: ARNT interacting protein, ARNT-interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, hypoxia-inducible factor1alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1, PASD8.
• NCBI Gene Id: 3091
• Protein Name: Hypoxia-inducible factor 1-alpha
• Description of Target: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
• Uniprot ID: Q16665
• Protein Accession #: NP_001230013.1
• Nucleotide Accession #: NM_001243084.1
• Molecular Weight: 93 kDa