HIF1 alpha Antibody : Biotin (OASE00130-Biotin)

Catalog No: OASE00130-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: ESEE122
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: ICC, IF, IHC, WB, ELISA
• Additional Information: Background Info: Specific for HIF1Alpha
  Scientific Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the
  cellular response of hypoxia (1). The HIF1 complex
  consists of two subunits, HIF1-Alpha and HIF1-Beta,
  which are basic helix-loop-helix proteins of the PAS
  family (2). HIF1 regulates the transcription of a broad
  range of genes that facilitate responses to the hypoxic
  environment, including genes regulating angiogenesis,
  erythropoiesis, cell cycle, metabolism and apoptosis. The
  widely expressed HIF-1alpha is typically degraded rapidly in
  normoxic cells by the ubiquitin/proteasomal pathway.
  Under normoxic conditions, HIF-1alpha is proline
  hydroxylated leading to a conformational change that
  promotes binding to the von Hippel Lindau protein (VLH)
  E3 ligase complex; ubiquitination and proteasomal
  degradation follows (3, 4). Both hypoxic conditions and
  chemical hydroxylase inhibitors (such as desferrioxamine
  and cobalt) inhibit HIF-1alpha degradation and lead to its
  stabilization. In addition, HIF-1alpha can be induced in an
  oxygen-independent manner by various cytokines through
  the PI3K-AKT-mTOR pathway (5-7).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of HIF1alpha in 20ug of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Recombinant fragment corresponding to amino acids 329-530
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000-1:8000 (IHC), 8-21ug/mL (ICC/IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5, 437-48.
  2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92,
  5510-4.
  3. Jaakkola P., et al. (2001) Science 292, 468-72.
  4. Maxwell P.H., et al. (1999) Nature 399, 271-5.
  5. Fukuda R., et al. (2002) J Biol Chem 277, 38205-11.
  6. Jiang B.H., et al. (2001) Cell Growth Differ 12, 363-
  9.
  7. Laughner E., et al. (2001) Mol Cell Biol 21, 3995-
  4004.

Target Info

• Gene Symbol: Hif1a
• Gene Full Name: hypoxia inducible factor 1, alpha subunit
• Alias Symbols: MO, HIF1, MOP1, HIF-1, bHLHe7, bHLHe78, AA959795, HIF1alpha, HIF1-alpha, HIF-1-alpha
• NCBI Gene Id: 15251
• Uniprot ID: Q61221
• Protein Accession #: NP_034561.2