HHIP ELISA Kit (Mouse) (OKCD08175)

Catalog No: OKCD08175

Size:96WELLS

HHIP ELISA Kit (Mouse) (OKCD08175) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Interacting Protein (HHIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Interacting Protein (HHIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Interacting Protein (HHIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hedgehog Interacting Protein (HHIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Interacting Protein (HHIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Interacting Protein (HHIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HHIP Microplate: 96 Wells (12 x 8 Well strips)
  • HHIP Lyophilized Standard: 2
  • 100X HHIP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.062ng/mL

Target Info

• Gene Symbol: HHIP
• Gene Full Name: Hedgehog-interacting protein
• Alias Symbols: H, Hi, Hhi, Hhip1
• NCBI Gene Id: 15245
• Protein Name: hedgehog-interacting protein
• Description of Target: Modulates hedgehog signaling in several cell types, including brain and lung through direct interaction with members of the hedgehog family. soluble forms inhibit shh-induced differentiation in the fibroblast cell line c3h/10t1/2.
• Uniprot ID: Q7TN16
• Protein Accession #: NP_064655.4
• Nucleotide Accession #: NM_020259.4