HHIP Antibody (OAGA00979)

Catalog No: OAGA00979

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
OAGA00979 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00979 diluted at 1:1000

HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HHIP antibody [N3C2], Internal (OAGA00979) dilution: 1:500.

HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min.
Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody (OAGA00979) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.

Product Info

• Predicted Species Reactivity: Human, Mouse, Chicken
• Product Format: Liquid 1X PBS with 20% Glycerol (pH 7). 0.025% ProClin300 was added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
  Positive Controls: mouse brain
  Cellular Localization: Cell membrane, peripheral membrane protein, secreted, isoform 2: cytoplasm
  Cellular Localization: Cell membrane; Peripheral membrane protein (By similarity), Secreted (By similarity), Isoform 2: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 263 and 554 of HHIP (Uniprot ID#Q96QV1)
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Rat (98%), Xenopus laevis (83%), Rhesus Monkey (100%), Bovine (98%)
• Concentration: 1.34 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  WB: 1:500~3000
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: HHIP
• Gene Full Name: Hedgehog-interacting protein
• Alias Symbols: HIP
• NCBI Gene Id: 64399
• Protein Name: Hedgehog-interacting protein
• Description of Target: This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.
• Uniprot ID: Q96QV1
• Protein Accession #: NP_071920.1
• Molecular Weight: 79 kDa