Catalog No: OCOA13977
Size:20UG
Price: $179.00
SKU
OCOA13977
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HHEX Protein Lysate (OCOA13977)

Datasheets/ManualsPrintable datasheet for HHEX Protein Lysate (OCOA13977)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionHHEX HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolHHEX
Gene Full Namehematopoietically expressed homeobox
Alias Symbolshematopoietically-expressed homeobox protein HHEX;HEX;HMPH;homeobox protein HEX;homeobox protein PRH;homeobox, hematopoietically expressed;HOX11L-PEN;PRH;PRHX;proline-rich homeodomain-containing transcription factor.
NCBI Gene Id3087
Protein NameNM_002729, NP_002720, Q03014
Description of TargetHematopoietically-expressed homeobox protein HHEX, hematopoietically-expressed homeobox protein HHEX [Homo sapiens], Homo sapiens hematopoietically expressed homeobox (HHEX), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q03014
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_002720
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_002729
Molecular Weight29.8 kDa
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