HHAT Antibody - N-terminal region (OAAB02917)

Catalog No: OAAB02917

Size:400ul

HHAT Antibody (N-term) western blot analysis in K562 cell lysates (35ug/lane).This demonstrates the HHAT antibody detected the HHAT protein (arrow).

HHAT Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHAT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HHAT Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
• Additional Information: Function: Catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling (By similarity). May bind GTP.
  Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein
  Tissue Location Ubiquitously expressed in normal tissue and cancer cell lines
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human HHAT.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: HHAT Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: HHAT
• Alias Symbols: Skn, NNMS, SKI1, MART2
• NCBI Gene Id: 55733
• Protein Name: Protein-cysteine N-palmitoyltransferase HHAT
• Description of Target: HHAT encodes an enzyme that acts within the secretory pathways to catalyze amino-terminal palmitoylation of SHH (see MIM 600725).
• Uniprot ID: Q5VTY9
• Protein Accession #: NP_001116306.1
• Molecular Weight: 57313 Da