HGS Antibody (OAAB18365)

Catalog No: OAAB18365

Size:100ul

Western blot analysis of lysate from Hela cell line, using HGS Antibody.
OAAB18365 was diluted at 1:1000.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysate at 35ug.
Dilution: 1:1000

Immunohistochemistry analysis of paraffin-embedded human testis tissue using HGS antibody.
Dilution: 1:50-1:100

Immunofluorescence analysis of HUVEC cells, using HGS antibody.
Dilution: 1:100

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IF, IHC
  Cellular Location: Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome, multivesicular body membrane; Peripheral membrane protein. Note=Colocalizes with UBQLN1 in ubiquitin- rich cytoplasmic aggregates that are not endocytic compartments.
  Tissue Location: Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Concentration: 0.5 mg/ml
• Application Info: WB: 1:1000
  IF: 1:100
  IHC: 1:50~1:100
• Reference: Asao H., et al.J. Biol. Chem. 272:32785-32791(1997).
  Lu L., et al.Gene 213:125-132(1998).
  Scoles D.R., et al.Hum. Mol. Genet. 9:1567-1574(2000).
  Kalnine N., et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
  Komada M., et al.J. Biol. Chem. 272:20538-20544(1997).

Target Info

• Gene Symbol: HGS
• Alias Symbols: HRS
• NCBI Gene Id: 9146
• Description of Target: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down- regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
• Uniprot ID: O14964
• Molecular Weight: 86 kDa