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Catalog No: OKCD07886
Size:96WELLS
Price: $575.00
SKU
OKCD07886
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Datasheets/ManualsPrintable datasheet for HFE2 ELISA Kit (Mouse) (OKCD07886)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemojuvelin (HJV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemojuvelin (HJV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemojuvelin (HJV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hemojuvelin (HJV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemojuvelin (HJV) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemojuvelin (HJV) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
HFE2 Microplate96 Wells (12 x 8 Well strips)
HFE2 Lyophilized Standard2
100X HFE2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.256ng/mL
Gene SymbolHjv
Gene Full Namehemojuvelin BMP co-receptor
Alias Symbols2310035L15Rik;5230400G09Rik;AI414844;AI789733;DL-;DL-M;hemochromatosis type 2 (juvenile) (human homolog);hemochromatosis type 2 protein homolog;hemoju;hemojuvelin;hemojuvelin BMP coreceptor;Hfe2;HJ;repulsive guidance molecule C;Rgm;RGM domain family member C;Rgmc.
NCBI Gene Id69585
Protein NameHemojuvelin
Description of TargetActs as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.
Uniprot IDQ7TQ32
Protein Accession #NP_081402.3
Nucleotide Accession #NM_027126.4
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