HES1 Antibody - N-terminal region : HRP (ARP32372_T100-HRP)

Catalog No: ARP32372_T100-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: IF, IP, WB, IHC
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HES1
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-HES1 (ARP32372_T100-HRP) antibody is Catalog # AAP32372 (Previous Catalog # AAPP03361)
• Sample Type Confirmation:

  HES1 is strongly supported by BioGPS gene expression data to be expressed in Raji

• Reference: Hoebeke,I., et al., (2006) Blood 107 (7), 2879-2881
• Publications:

  Tilley, A. E. et al. Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 179, 457-66 (2009). ChIP, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 19106307

  Schreck, K. C. et al. The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance. Clin. Cancer Res. 16, 6060-70 (2010). WB, ChIP, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 21169257

  Ma, W. et al. hCLP46 regulates U937 cell proliferation via Notch signaling pathway. Biochem. Biophys. Res. Commun. 408, 84-8 (2011). WB, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 21458412

  Bennani-Baiti, I. M. et al. Notch signalling is off and is uncoupled from HES1 expression in Ewing’s sarcoma. J. Pathol. 225, 353-63 (2011). IHC, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 21984123

  Asnaghi, L. et al. Notch signaling promotes growth and invasion in uveal melanoma. Clin. Cancer Res. 18, 654-65 (2012). ChIP, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 22228632

  Guiu, J. et al. Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling. J. Exp. Med. 210, 71-84 (2013). ChIP, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 23267012

  Chu, Q., Liu, L. & Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46, 254-62 (2013). WB, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 23692084

  Ma, L. et al. Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits. Biochem. Biophys. Res. Commun. 450, 353-9 (2014). WB, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 24944018

  Asnaghi, L. et al. Hypoxia Promotes Uveal Melanoma Invasion through Enhanced Notch and MAPK Activation. PLoS One 9, e105372 (2014). WB, Human, Rat, Pig, Zebrafish, Horse, Rabbit, Bovine, Guinea pig, Mouse 25166211

Target Info

• Gene Symbol: HES1
• Gene Full Name: Hairy and enhancer of split 1, (Drosophila)
• Alias Symbols: HHL, HRY, HES-1, bHLHb39
• NCBI Gene Id: 3280
• Protein Name: Transcription factor HES-1
• Description of Target: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
• Uniprot ID: Q14469
• Protein Accession #: NP_005515
• Nucleotide Accession #: NM_005524
• Protein Size (# AA): 280
• Molecular Weight: 30kDa
• Protein Interactions: STAT3; JAK2; APH1A; LTBR; ASGR2; TLE1; ELAVL1; PARP1; UBC; NUDT3; HDAC6; YWHAB; PTK2; NR4A1; HMGCL; GAPDH; FHL1; CSNK1E; APCS; FANCE; FANCC; FANCA; FANCL; SPTAN1; HSP90AA1; HSPA4; FANCG; FANCF; HDAC1; RUNX2; HES6; ID4; ID1; SIRT1; UBQLN1; HEY2; ID2; ID3;