HES1 Antibody - N-terminal region (ARP32372_T100)

Catalog No: ARP32372_T100

WB Suggested Anti-HES1 Antibody Titration: 1.25ug/ml
Positive Control: Raji cell lysateHES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells

HES1 antibody - N-terminal region (ARP32372_T100) validated by WB using transfected Ls174Tcell lysate at 1ug/ml.

HES1 antibody - N-terminal region (ARP32372_T100) validated by WB using Mouse embryo tissue at 1:800.

Rabbit Anti-HES1 Antibody
Catalog Number: ARP32372
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

transfected Ls174T cell lysate at 50ug/ml.

K562

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IF, IP, WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HES1
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-HES1 (ARP32372_T100) antibody is Catalog # AAP32372 (Previous Catalog # AAPP03361)
• Sample Type Confirmation:

  HES1 is strongly supported by BioGPS gene expression data to be expressed in Raji

• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Reference: Hoebeke,I., et al., (2006) Blood 107 (7), 2879-2881
• Publications:

  A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal. Dis Model Mech. 12, (2019). 30910991

  Asnaghi, L. et al. Hypoxia Promotes Uveal Melanoma Invasion through Enhanced Notch and MAPK Activation. PLoS One 9, e105372 (2014). 25166211

  Asnaghi, L. et al. Notch signaling promotes growth and invasion in uveal melanoma. Clin. Cancer Res. 18, 654-65 (2012). 22228632

  Bennani-Baiti, I. M. et al. Notch signalling is off and is uncoupled from HES1 expression in Ewing’s sarcoma. J. Pathol. 225, 353-63 (2011). 21984123

  CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A. Oncogene. 37, 128-138 (2018). 28892048

  Chu, Q., Liu, L. & Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46, 254-62 (2013). 23692084

  Guiu, J. et al. Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling. J. Exp. Med. 210, 71-84 (2013). 23267012

  Ma, L. et al. Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits. Biochem. Biophys. Res. Commun. 450, 353-9 (2014). 24944018

  Ma, W. et al. hCLP46 regulates U937 cell proliferation via Notch signaling pathway. Biochem. Biophys. Res. Commun. 408, 84-8 (2011). 21458412

  Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2. Neuroscience. 306, 100-14 (2015). 26299339

  Schreck, K. C. et al. The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance. Clin. Cancer Res. 16, 6060-70 (2010). 21169257

  Targeting Notch Signaling and Autophagy Increases Cytotoxicity in Glioblastoma Neurospheres. Brain Pathol. 26, 713-723 (2016). 26613556

  Therapeutic targeting of HES1 transcriptional programs in T-ALL. Blood. 125, 2806-14 (2015). 25784680

  Tilley, A. E. et al. Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 179, 457-66 (2009). 19106307

• Description:
  View product citations for HES1 Antibody - N-terminal region (ARP32372_T100) on CiteAb

Target Info

• Gene Symbol: HES1
• Gene Full Name: Hairy and enhancer of split 1, (Drosophila)
• Alias Symbols: HHL, HRY, HES-1, bHLHb39
• NCBI Gene Id: 3280
• Protein Name: Transcription factor HES-1
• Description of Target: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
• Uniprot ID: Q14469
• Protein Accession #: NP_005515
• Nucleotide Accession #: NM_005524
• Protein Size (# AA): 280
• Molecular Weight: 30 kDa
• Protein Interactions: STAT3; JAK2; APH1A; LTBR; ASGR2; TLE1; ELAVL1; PARP1; UBC; NUDT3; HDAC6; YWHAB; PTK2; NR4A1; HMGCL; GAPDH; FHL1; CSNK1E; APCS; FANCE; FANCC; FANCA; FANCL; SPTAN1; HSP90AA1; HSPA4; FANCG; FANCF; HDAC1; RUNX2; HES6; ID4; ID1; SIRT1; UBQLN1; HEY2; ID2; ID3;