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HES1 Antibody - N-terminal region (ARP32372_T100)

100 ul
$249.00
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Conjugation Options

ARP32372_T100-FITC Conjugated

ARP32372_T100-HRP Conjugated

ARP32372_T100-Biotin Conjugated

Gene Symbol:
HES1
Official Gene Full Name:
Hairy and enhancer of split 1, (Drosophila)
NCBI Gene Id:
3280
Protein Name:
Transcription factor HES-1
Swissprot Id:
Q14469
Protein Accession #:
NP_005515
Nucleotide Accession #:
NM_005524
Alias Symbols:
HHL, HRY, HES-1, bHLHb39
Replacement Item:
This antibody may replace item sc-120760 from Santa Cruz Biotechnology.
Description of Target:
HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Protein Size (# AA):
280
Molecular Weight:
30kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Protein A purified
Application:
IF, IP, WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HES1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HES1.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human HES1
Predicted Species Reactivity:
Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Tested Species Reactivity:
Human, Mouse
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data:
Anti-HES1 (ARP32372_T100)
Peptide Sequence:
Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
STAT3; JAK2; APH1A; LTBR; ASGR2; TLE1; ELAVL1; PARP1; UBC; NUDT3; HDAC6; YWHAB; PTK2; NR4A1; HMGCL; GAPDH; FHL1; CSNK1E; APCS; FANCE; FANCC; FANCA; FANCL; SPTAN1; HSP90AA1; HSPA4; FANCG; FANCF; HDAC1; RUNX2; HES6; ID4; ID1; SIRT1; UBQLN1; HEY2; ID2; ID3;
Blocking Peptide:
For anti-HES1 (ARP32372_T100) antibody is Catalog # AAP32372 (Previous Catalog # AAPP03361)
Datasheets/Manuals:
Printable datasheet for anti-HES1 (ARP32372_T100) antibody
Sample Type Confirmation:

HES1 is strongly supported by BioGPS gene expression data to be expressed in Raji

Target Reference:
Hoebeke,I., et al., (2006) Blood 107 (7), 2879-2881
Publications:

Asnaghi, L. et al. Hypoxia Promotes Uveal Melanoma Invasion through Enhanced Notch and MAPK Activation. PLoS One 9, e105372 (2014). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 25166211

Asnaghi, L. et al. Notch signaling promotes growth and invasion in uveal melanoma. Clin. Cancer Res. 18, 654-65 (2012). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 22228632

Bennani-Baiti, I. M. et al. Notch signalling is off and is uncoupled from HES1 expression in Ewing’s sarcoma. J. Pathol. 225, 353-63 (2011). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 21984123

Chu, Q., Liu, L. & Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46, 254-62 (2013). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 23692084

Guiu, J. et al. Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling. J. Exp. Med. 210, 71-84 (2013). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 23267012

Ma, L. et al. Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits. Biochem. Biophys. Res. Commun. 450, 353-9 (2014). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 24944018

Ma, W. et al. hCLP46 regulates U937 cell proliferation via Notch signaling pathway. Biochem. Biophys. Res. Commun. 408, 84-8 (2011). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 21458412

Meng, S; Su, Z; Liu, Z; Wang, N; Wang, Z; Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2. 306, 100-14 (2015). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 26299339

Natsumeda, M; Maitani, K; Liu, Y; Miyahara, H; Kaur, H; Chu, Q; Zhang, H; Kahlert, UD; Eberhart, CG; Targeting Notch Signaling and Autophagy Increases Cytotoxicity in Glioblastoma Neurospheres. 26, 713-723 (2016). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 26613556

Schreck, K. C. et al. The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance. Clin. Cancer Res. 16, 6060-70 (2010). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 21169257

Tilley, A. E. et al. Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 179, 457-66 (2009). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 19106307

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63/03/2019 20:18
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  • Quality:
HEK293 cell lysates in WB

Submitted by: Anonymous

HES1

Host: Rabbit

Lane 1: 40ug HEK293 cell lysate

Lane 2: 40ug HEK293 cell lysate

Primary antibody dilution: 1:1000

Secondary antibody dilution: 1:3000

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